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KMID : 1005219930080010031
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Journal of Embryo Transfer
1993 Volume.8 No. 1 p.31 ~ p.36
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Post-thaw Survival of Mouse Embryos of Various Developmental Stages Cryopreserved by Vitrification in Ethylene Glycol-Based Solution
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정기화
공일근/박준규/곽대오/박충생
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Abstract
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The post-thaw survival of mouse embryos of the various developmental stages was determined after cryopreservation by vitrification in a solution containing ethylene glycol, Ficoll and sucrose (EFS). All the embryos were equilibrated for 2 minutes just prior to freezing. The number of blastomeres during in vitro development was counted by nuclei higher rates of post-thaw survival were obtained from the embryos of 2-cell(92.2%), 8-cell(77.2%) or morula stage(90.0%) than those of blastocyst stage(62.7%). The number of blastomeres per embryo following in vitro culture for 24 hours was significantly(P<0.05) smaller as 66.0f22.3 in vitrified and thawed morulae than fresh morulae(91.7±12.2).
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KEYWORD
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